02.08. Área de Salud VIII Mar Menor

Implementation of quantitative HBsAg testing in clinical microbiology laboratories in Spain: Results from a national GEHEP-SEIMC survey

Sat, 01 Aug 2026 00:00:00 GMT

Implementation of quantitative HBsAg testing in clinical microbiology laboratories in Spain: Results from a national GEHEP-SEIMC survey Chaves-Blanco, Lucía; Illescas-López, Marta; Fuentes, Ana; Carracedo, Raquel; Delgado-Iribarren, Alberto; Vázquez-Blanquino, Alberto; Blázquez, Ana-María; Saez, Ruth; Miqueleiz, Ana; de-los-Reyes-Vidal, María; Tosco, Tomas; Gómez, César; Moldes, Luz; Calvo, Noelia; Pérez-Rodríguez, Lucía; Trigo, Matilde; Dolores-Ocete, María; Alcaraz, María-Jesús; Asuncion-Iborra, María; García, Julio; Joaquin-Blas, José; María-Vallejo, Aldara; Alberola-Romano, Ana; Illescas, Soledad; Rodríguez, Juan-Carlos; Alonso, Roberto; Cámara, Margarita; Dolores-Navarro, María; Luisa-Núñez, María; del-Amor-Espín, María-Jesús; Beteta, Alicia; Cascales-Alcolea, Eva; Miguel-Soria, Luis; Gacinuno, Sonsoles; Sandoval, Marta; Cortizo, Sandra; Ordóñez, Patricia; Liendo, Paloma; Pérez, Mercedes; Álvarez-Arguelles, Marta; García, Fernando; Rodríguez, Inmaculada; Gómez, Carmen; Elorduy, Luis; Hernández-Febles, Melisa; González-Praetorius, Alejandro; Liebana, Carmen; Lourdes-Molina, María; Salicio, Yolanda; Macho, Mikele; Becerril, Berta; Franco-Álvarez, Francisco; Falcés-Romero, Iker; Algarete, Sonia; Jose-Gude, María; Pablo, Daniel; Magdalena-Lara, María; Montiel, Natalia; Freyre, Carolina; Galán, Juan-Carlos; Pérez, Ana-Belén; Lorenzo, Belén; Fraile, Pablo; Sánchez-Yebra, Waldo; Viciana, Isabel; Sampedro, Antonio; del-Carmen-Lozano, María; Domínguez-Castano, Ana; Ramírez, Encarnación; Beltran, Inocencio; Martin, Teresa; Puerta, Antonio; Tajada, Pilar; Panes, Paula; Martín, Adrián; Hurtado, Juan-Carlos; López-Braos, Javier; Arjona, Francisco; Miro, Elisenda; Ruiz, Montserrat; Ramírez, Mercedes; Gil-Fortuno, María; González-Pellicer, Rosa; Acedo, Juan-Manuel; Anel-Pedroche, Jorge; Infante-Urrios, Ana; Domínguez, Victoria; Aran-Toha, Guillermo; Mrtinez-Macias, Olalla; Orta-Mira, Nieves; Torres-Sopena, Luis; Cantudo, Purificación; Araceli-Hernández, María; Arroyo-Serrano, Teresa; Rando, Ariadna; Garrido, Marta; Fernández, Gema; de-Salazar, Adolfo; Aguilera, Antonio; García, Federico

Surgical Management and Therapeutic Strategy of Uterine Sarcoma According to Histological Subtype and Staging: Updated Review and Recommendations

Mon, 08 Jun 2026 00:00:00 GMT

Surgical Management and Therapeutic Strategy of Uterine Sarcoma According to Histological Subtype and Staging: Updated Review and Recommendations Muñoz-Casares, Francisco-Cristobal; Gómez-Barbadillo, José; Álvarez-Álvarez, Rosa; Hindi, Nadia; Sebio, Ana; Lozano-Lominchar, Pablo; Fernández-Hernández, Juan-Ángel; Vasques, Hugo; Muñoz-Muñoz, Paula; Asencio-Pascual, José-Manuel BACKGROUND: Uterine sarcomas represent a small and heterogeneous subgroup of uterine malignancies (accounting for less than 5%), characterized by high biological aggressiveness, high recurrence rates, and diagnostic complexity; surgical management remains the cornerstone of treatment and, consequently, the primary determinant of patient prognosis. OBJECTIVE: This review synthesizes current evidence regarding the accurate diagnosis and appropriate surgical management of the main histological subtypes of uterine sarcoma across different stages, within a multidisciplinary therapeutic framework. METHODS: A comprehensive narrative review was conducted using recent publications from major biomedical databases, with an emphasis on studies exploring surgical outcomes, molecular profiling, therapeutic strategies, and survival patterns. RESULTS: Leiomyosarcoma and endometrial stromal sarcoma represent more than 80% of histological subtypes, with histological grade being the factor of greatest prognostic significance. Complete disease resection, without tumor fragmentation and with negative surgical margins, is the undisputed standard objective. Variations from the standard treatment-en bloc total hysterectomy with bilateral salpingo-oophorectomy-exist depending on histology and tumor staging. CONCLUSIONS: Individualized surgical treatment tailored to the specific histological subtype, combined with multimodal treatment strategies based on accurate tumor staging, molecular characterization, and recurrence patterns, is essential to improve survival. Such management should be conducted in referral centers with expertise in treating these rare and fearsome malignancies.

Auditory brainstem response in pigs: normative data and insights from TPC2 knockout models

Wed, 01 Jul 2026 00:00:00 GMT

Auditory brainstem response in pigs: normative data and insights from TPC2 knockout models Templado, Sheila; Almela-Rojo, Maite; Barcelo, María-Dolores; Espinosa-Fernández, Laura; Pineiro-Silva, Celia; Navarro-Serna, Sergio; Parrington, John; García-Purrinos, Francisco-José; Gadea-Mateos, Joaquín BACKGROUND: Pigs are increasingly used as translational models for auditory research due to anatomical and physiological similarities with humans. However, normative auditory brainstem response (ABR) parameters in commercial pig breeds remain poorly characterized, limiting their use in hearing research and genetic models. OBJECTIVE: To establish reference ABR values across stimulus intensities in pigs and assess the influence of genotype, age, and sex, including the evaluation of TPC2 knockout (KO) animals. METHODS: ABR recordings were obtained from 42 pigs (wild-type, heterozygous, and TPC2KO) under under controlled anesthetic conditions. Click stimuli (90-10 dB nHL) were presented via insert earphones. Latencies and amplitudes of waves I-VI, interpeak intervals, and detection thresholds were analyzed using repeated-measures ANOVA and linear mixed-effects models. Machine learning approaches were used exploratorily to assess potential non-linear relationships among variables. RESULTS: Peak detection and amplitude decreased with lower intensities (P < 0.001), while latencies increased. Wave V remained detectable at all intensities, with a mean threshold of 33.6 ± 2.7 dB nHL. No significant differences were observed between ears or across genotypes and sex. Age influenced the I-III interpeak interval (P < 0.05) but not individual wave latencies or amplitudes. Machine learning analyses identified age as the most relevant contributor to ABR variability among tested factors. CONCLUSION: This study provides normative ABR reference values for pigs, defining normative values across intensities and demonstrates the absence of detectable click-evoked ABR alterations in TPC2KO animals under the present conditions. These findings support the use of pigs as robust models for auditory research and genetic studies of hearing loss.

Molecular epidemiology of Dientamoeba fragilis coinfections with Blastocystis sp. and Giardia duodenalis in symptomatic patients from Valencia, Spain

Thu, 09 Jul 2026 00:00:00 GMT

Molecular epidemiology of Dientamoeba fragilis coinfections with Blastocystis sp. and Giardia duodenalis in symptomatic patients from Valencia, Spain García-Hita, Marta; Ferriz, Jorge; Cifre, Susana; Tapia-Veloz, Gabriela; Molina, Araceli; Carmena, David; Trelis, María PURPOSE: Dientamoeba fragilis is a cosmopolitan intestinal protist with an insufficiently understood life cycle and pathogenicity. Diagnosis by light microscopy is challenging, leading to significant underdiagnosis. Two genotypes (1 and 2) have been described, with genotype 1 being the most frequently detected worldwide. As no molecularly characterized isolates from Spain have been reported, we performed a molecular characterization and evaluated sociodemographic factors of D. fragilis in patients coinfected with Blastocystis sp. and/or Giardia duodenalis. METHODS: Using a multiplex qPCR assay (Allplex? GI-Parasite Assay, Seegene), we analyzed 354 stool samples previously identified as positive for Blastocystis sp. (n = 276), G. duodenalis (n = 63), or both (n = 15) by light microscopy. Eligible positive samples were selected for conventional PCR targeting the SSU rRNA gene and subsequent Sanger sequencing. RESULTS: D. fragilis was detected in 34.5% of samples by qPCR; 32.3% of Blastocystis-positive, 44.9% of Giardia-positive and 46.7% of coinfected samples. We successfully sequenced 22 isolates, all corresponding to genotype 1, identifying two distinct intra-genotypic sequence variants. Younger age was positively associated with D. fragilis, with individuals coinfected with Blastocystis sp. being markedly younger than those monoinfected. Patients with G. duodenalis and D. fragilis were mainly under 15 years of age. CONCLUSION: This study provides the first genotyping of D. fragilis in Spain, demonstrating the circulation of two genotype 1 variants. Findings highlight the underdiagnosis of D. fragilis and its high prevalence in young patients with other fecal-orally transmitted protists. Evaluating coinfections is crucial to understanding parasite relationships and should be considered for clinical management, especially when symptoms persist.